Denaturation mapping studies on the circular chloroplast deoxyribonucleic acid from pea leaves.
نویسندگان
چکیده
The structure of circular pea chloroplast DNA (ctDNA) has been analyzed by denaturation mapping. All of the pea ctDNA molecules that were examined had identical gross base sequences. Denaturation maps were constructed at denaturation levels of 2.5%, 22%, and 44%. These denaturation maps showed that the circular pea ctDNA contained six small AT-rich regions on one-half of the DNA molecule, and two small GC-rich regions on the other half of the DNA molecule. The structure of pea ctDNA circular dimers was also examined. The results showed that the pea ctDNA circular dimers consisted of two monomer length units integrated in tandem repeat.
منابع مشابه
Molecular size and conformation of chloroplast deoxyribonucleic acid from pea leaves.
The molecular size and conformation of chloroplast (ct-) DNA has been studied by renaturation kinetics and electron microscopy. Ct-DNA of buoyant density 1.698 f 0.001 g per cm3 showed a T,, of 84” and a homogeneous melting pattern. Denatured and sheared c&DNA renatured as a single kinetic class with no suggestion of repeating sequences. Ct-DNA is found to have a molecular weight of 95 x lo6 by...
متن کاملConformation of chloroplast DNA in wheat leaves
Sedimentation and electron microscopic studies have provided no evidence for the presence of circular DNA (deoxyribonucleic acid) in chloroplasts from wheat leaves. Electron microscopy of osmotically disrupted chloroplasts has revealed the presence of linear duplex filaments, some with a tertiary helicoidal configuration and possibly monostranded filaments. None of these structures was observed...
متن کاملHybridization of leucyl-transfer ribonucleic Acid isoacceptors from green leaves with nuclear and chloroplast deoxyribonucleic Acid.
Chromatographically distinct isoacceptors of leucyl-tRNA from mature bean leaves were all observed to hybridize with bean chloroplast and nuclear DNA in a ratio similar to that exhibited by the unfractionated leucyl-tRNA. Under the same hybridization conditions, maize tRNA failed to form a stable hybrid with bean DNA, and levels of hybridization between bean-leaf leucyl-tRNA and nuclear or chlo...
متن کاملAntigenic relationships between chloroplast and cytosolic fructose-1,6-bisphosphatases.
Cytosolic fructose-1,6-biphosphatases (FBPase, EC 3.1.3.11) from pea (Pisum sativum L. cv Lincoln) and spinach (Spinacia oleracea L. cv Winter Giant) did not cross-react by double immunodiffusion and western blotting with either of the antisera raised against the chloroplast enzyme of both species; similarly, pea and spinach chloroplast FBPases did not react with the spinach cytosolic FBPase an...
متن کاملNucleotide sequence analysis of a cDNA encoding chloroplastic fructose-1,6-bisphosphatase from pea (Pisum sativum l.).
Fructose-1,6-bisphosphatase (EC 3.1.3.11) catalyzes the dephosphoric reaction of Fru-1,6-bis P to Fru-6-P and Pi. Two isozymes exist in higher plants, one in the cytosol and the other in the chloroplast. The latter is a pivotal enzyme for photosynthetic carbon dioxide assimilation. It is active in the light but almost inactive in darkness. The lightmediated activation of the enzyme is attribute...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- The Journal of biological chemistry
دوره 250 13 شماره
صفحات -
تاریخ انتشار 1975